Journal: Cell metabolism

Article Title: Ketogenic diet promotes tumor ferroptosis but induces relative corticosterone deficiency that accelerates cachexia

doi: 10.1016/j.cmet.2023.05.008

Figure Lengend Snippet: Key resources table

Article Snippet: Progression Free Survival Mice were considered to have reached the PFS endpoint when the volume of their tumors exceeded 2000 mm 3 , as measured by handheld calipers. . Dexamethasone treatment Dexamethasone 21-phosphate disodium salt (#D1159; Sigma-Aldrich) was dissolved in dH 2 O and administered intraperitoneally (i.p.) at 1mg/kg daily. . N-acetyl cysteine (NAC) treatment N-Acetyl-L-cysteine (#A9165; Sigma-Aldrich) was dissolved in 0.9% NaCl sterile saline solution (#Z1377; Thermo Fisher) and administered intraperitoneally (i.p.) at 150mg/kg daily. . Liproxstatin-1 treatment Liproxstatin-1 (#S7699; Selleck Chemicals) was dissolved in 2% Dimethyl Sulfoxide (DMSO) (#12611S; Cell Signaling), 40% Polyethylene glycol 300 (PEG300) (#S6704; Selleck Chemicals), 2% Tween-80 and 56% double-distilled water (ddH 2 O), individually and in this specific order, and administered intraperitoneally (i.p.) at 10mg/kg daily. . RSL3 treatment RSL3 ((1S,3R)-RSL3) (#HY-100218A; MedChemExpress) was dissolved in 10% DMSO (#12611S; Cell Signaling), 40% PEG300 (#S6704; Selleck Chemicals), 5% Tween-80 and 45% NaCl 0.9% sterile saline solution (#Z1377; Thermo Fisher) and administered intraperitoneally (i.p.) at 5mg/kg daily. . ACTH stimulation test (synacthen test) ACTH (#HOR-279, ProSpec) was reconstituted in dH 2 0 and injected intraperitoneally at a dose of 1 ug/g body weight.

Techniques: Recombinant, Sterility, Saline, Lysis, Protease Inhibitor, Red Blood Cell Lysis, Enzyme-linked Immunosorbent Assay, Iron Assay, Competitive ELISA, Software

Journal: Cell metabolism

Article Title: Ketogenic diet promotes tumor ferroptosis but induces relative corticosterone deficiency that accelerates cachexia

doi: 10.1016/j.cmet.2023.05.008

Figure Lengend Snippet: Key resources table

Article Snippet: Progression Free Survival Mice were considered to have reached the PFS endpoint when the volume of their tumors exceeded 2000 mm 3 , as measured by handheld calipers. . Dexamethasone treatment Dexamethasone 21-phosphate disodium salt (#D1159; Sigma-Aldrich) was dissolved in dH 2 O and administered intraperitoneally (i.p.) at 1mg/kg daily. . N-acetyl cysteine (NAC) treatment N-Acetyl-L-cysteine (#A9165; Sigma-Aldrich) was dissolved in 0.9% NaCl sterile saline solution (#Z1377; Thermo Fisher) and administered intraperitoneally (i.p.) at 150mg/kg daily. . Liproxstatin-1 treatment Liproxstatin-1 (#S7699; Selleck Chemicals) was dissolved in 2% Dimethyl Sulfoxide (DMSO) (#12611S; Cell Signaling), 40% Polyethylene glycol 300 (PEG300) (#S6704; Selleck Chemicals), 2% Tween-80 and 56% double-distilled water (ddH 2 O), individually and in this specific order, and administered intraperitoneally (i.p.) at 10mg/kg daily. . RSL3 treatment RSL3 ((1S,3R)-RSL3) (#HY-100218A; MedChemExpress) was dissolved in 10% DMSO (#12611S; Cell Signaling), 40% PEG300 (#S6704; Selleck Chemicals), 5% Tween-80 and 45% NaCl 0.9% sterile saline solution (#Z1377; Thermo Fisher) and administered intraperitoneally (i.p.) at 5mg/kg daily. . ACTH stimulation test (synacthen test) ACTH (#HOR-279, ProSpec) was reconstituted in dH 2 0 and injected intraperitoneally at a dose of 1 ug/g body weight.

Techniques: Recombinant, Sterility, Saline, Lysis, Protease Inhibitor, Red Blood Cell Lysis, Enzyme-linked Immunosorbent Assay, Iron Assay, Competitive ELISA, Software